Abstract
A simple rapid and stability-indicating LC method using a narrow-bore column has been developed, fully validated, and applied to the quantification of alfuzosin in pharmaceutical formulations. Chromatography was achieved isocratically on a narrow-bore, 5-lm particle size, C8 analytical column. The mobile phase was a 35:65 (v/v) 0.0125 M ammonium formate‐acetonitrile at a flow rate of 0.35 mL min � 1 . Detection was by UV absorption at 245 nm. Evaluation over the range 200‐800 ng mL � 1 revealed linearity was good. Limits of detection and quantification for alfuzosin were 22.9 and 69.5 ng mL � 1 , respectively. Intraday and inter-day RSD were less than 6.4%, and the relative percentage error was less than � 1.7% (n = 5). Accelerated degradation performed under different stress conditions including oxidation, hydrolysis, and heat, proved the selectivity of the procedure. The method was successfully used for quality-control and content-uniformity testing of commercial tablets.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
