Abstract
An improved method for cholesterol determination in egg yolk is reported in this paper. Egg yolk was first diluted. Cholesterol was then extracted with ether and petroleum ether, and quantified by reverse phase chromatography on a Zorbax ODS column (0.46 x 15 cm, 5-6 micrometer) using a mobile phase of acetonitrile and 2-propanol (4:1) with a flow rate of 0.6 mL/min. A linear correlation was observed between cholesterol concentration at 0.05-0.40 mg/mL and its peak heights with a correlation coefficient of 0.9993 (n = 5). The average recovery was 98.9%, and detection limit was 0.02 mg/mL. No differences in cholesterol concentration were observed between egg yolk samples with and without saponification. Rapid and reproducible quantification of cholesterol in egg yolk can be completed with this simplified method.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
