Abstract
The Schultz histochemical adaptation of the Burchard-Liebermann reaction is considered by most authors to be specific for cholesterol and its esters (2, 3). The latter are thought to respond positively to the test after being converted to 7-hydroxycholesterol or a compound very similar to it (4). There are three undesirable technical features associated with the Schultz test procedure when it is used as commonly described. First, there is frequently a precipitation of ferric ammonium sulfate on the tissue sections during incubation. Secondly, bubble formation often occurs after the coverslip is applied, many times regardless of the purity of the acetic-sulfuric acid mixture. Lastly, the intensity of color development is often quite variable from one area to another, especially when the amount of cholesterol present in the tissue is small. We have found these difficulties in the technique to be of considerable concern in our laboratory since ruminant adrenals, which we work with primarily, contain much less cholesterol than those of most other species.
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