Abstract
1. An improved method for the isolation of chlorophyll components a and b has been developed. The methods of WILLSTATTER and STOLL and of TSWETT were combined and modified. The final purification processes involve differential adsorption on talc and fractional precipitation from petroleum ether, for components a and b respectively. The steps of the procedure are presented in detail. 2. The absorption spectra of chlorophylls a and b were measured accurately from λ 3950-7800 A. by a photoelectric method. Absorption coefficients at the maxima and minima were determined with an accuracy of 1.0%. The visible spectrum of each component consists of seven bands. 3. Spectral data indicate that the components prepared by WILLSTATTER and STOLL and by TSWETT were not separated completely from one another. The spectral method for detection of impurities is discussed. 4. A comparative study was made of the colors of a and b in various solvents and of certain chlorophyll derivatives. 5. Different tests of purity are dis...
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.