Abstract
Abstract The four components of the genome of cowpea chlorotic mottle virus have been prepared in a highly active and highly purified state by a method based on their resolution by polyacrylamide gel electrophoresis. Activity and purity have been confirmed by gel electrophoresis under denaturing and non-denaturing conditions, infectivity tests on whole plants, translation in an mRNA dependent rabbit reticulocyte lysate and fingerprinting of T1 ribonuclease digests after labelling with 32P at the 5′ ends of the oligonucleotide products. The quality of the RNAs obtained was superior to those previously obtained by zonal centrifugation but only comparatively small batches of whole RNA can be handled with ease.
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