Abstract
A simple and reliable method for the quantitative determination of free tyrosine and. tyrosine residues in proteins is described. The method involves alkali-denaturation of proteins in a boiling water bath, the color forming reaction between tyrosine and 1-nitroso-2-naphthol in 19 N H2SO4, and measurement of the absorhance at 520 m μ. The method is preferable because of its simplicity, high sensitivity, reproducibility and remarkable stability of the red color. The procedure has been shown to be accurate with a wide variety of enzyme proteins.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
