Abstract
Our published method for determination of prostate concentrations of endogenous androgens has been modified to increase the precision, sensitivity and reliability. A more efficient tissue homogenizer (Tekmar) was used instead of the VirTis-driven blades. Silica gel adsorption of androgens in the solvent extract and subsequent separation from nonpolar lipids by selective solvent extraction in a batch process in tubes was substituted for column elution. Time, solvent and blanks were decreased. Cyproterone acetate, as a more reliable, internal relative R F marker on thin-layer chromatography (t.l.c.), replaced medrogestone. With increased sensitivity, better precision and ability to homogenize smaller tissue masses, our method was also suitable for scaling down from 1-g to biopsy-size (50 mg) specimens. The method appears to be quantitatively adequate for dihydrotestosterone (DHT), the most concentrated and biologically significant androgen in prostate, but present measurements of androstanediol ( 1 3 as concentrated as DHT in benign prostatic hypertrophy) are at least semi-quantitative.
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