Abstract
We examined the correlation between the tumorigenicity and the growth capacity in soft agar of various malignant cell lines. The colony forming rates were calculated in (a) 0%, (b) 0.15%, (c) 0.30%, and (d) 0.40% soft agar medium. An approximate value for the colony forming capacity, ‘y’, was then established for the formula\(y = \frac{1}{a}\int_0^a {f(x) dx} \) (x=concentration of soft agar, f(x)=rate of appearance of colony forming cells with x% soft agar medium).
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