Abstract
BackgroundSolanum lycopersicum, an economically important crop grown worldwide, has been used as a model for the study of arbuscular mycorrhizal (AM) symbiosis in non-legume plants for several years and several cDNA array hybridization studies have revealed specific transcriptomic profiles of mycorrhizal tomato roots. However, a method to easily screen candidate genes which could play an important role during tomato mycorrhization is required.ResultsWe have developed an optimized procedure for composite tomato plant obtaining achieved through Agrobacterium rhizogenes-mediated transformation. This protocol involves the unusual in vitro culture of composite plants between two filter papers placed on the culture media. In addition, we show that DsRed is an appropriate molecular marker for the precise selection of cotransformed tomato hairy roots. S. lycopersicum composite plant hairy roots appear to be colonized by the AM fungus Rhizophagus irregularis in a manner similar to that of normal roots, and a modified construct useful for localizing the expression of promoters putatively associated with mycorrhization was developed and tested.ConclusionsIn this study, we present an easy, fast and low-cost procedure to study AM symbiosis in tomato roots.
Highlights
Solanum lycopersicum, an economically important crop grown worldwide, has been used as a model for the study of arbuscular mycorrhizal (AM) symbiosis in non-legume plants for several years and several cDNA array hybridization studies have revealed specific transcriptomic profiles of mycorrhizal tomato roots
The aim of this work was to show the difficulties found for in vitro composite tomato plant obtaining and we set up a method to overcome these problems
We present a strategy for fast and efficient production of S. lycopersicum composite plants using A. rhizogenes MSU440, in which the transgenic roots of the composite plants showed the expression of the red fluorescence protein marker (DsRed)
Summary
An economically important crop grown worldwide, has been used as a model for the study of arbuscular mycorrhizal (AM) symbiosis in non-legume plants for several years and several cDNA array hybridization studies have revealed specific transcriptomic profiles of mycorrhizal tomato roots. A method to screen candidate genes which could play an important role during tomato mycorrhization is required. The Solanum lycopersicum (tomato) is an economically important crop grown worldwide. In addition to its cultivation and economic importance worldwide, S. lycopersicum has become a convenient model for research into plant genetics and physiology due to several features such as its relatively compact genome (950 Mb), the availability of a marker-saturated genetic linkage map and the annotated genome sequence The development of a procedure to screen candidate genes performing important functions during mycorrhization is required. Unlike A. tumefaciens, A. rhizogenes contains root locus (rol) genes, which, on induction, trigger the development of adventitious, genetically transformed so-called hairy roots due to the conspicuous abundance of their root hairs, a term first used in the literature by Stewart et al [13]
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