Abstract
An improved technique to prepare asbestos fibers collected on a cellulose-ester filter for phase contrast microscopy (PCM) is Presented. The filter is cleared with a solution of dimethyl formamide, acetic acid, and water. Gold electron microscope grids then are placed on the filter to define the area to be examined by PCM at 1250x. Compared with transmission electron microscopy of polycarbonate filters containing equivalent amounts of amosite asbestos fibers, approximately 106±12% of the fibers longer than 5 µm were detected by this technique. Excellent agreement also was obtained in the determination of the length and width distributions of the fibers. The relative standard deviation between the results obtained by the analysts in examining the same grid openings was found to he approximately 4.5% at a level of about 4 fibers per 0.01 mm2 of filter. The analyst errors in fiber enumeration were evaluated also.
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