An improved mass spectrometric method for identification and quantification of phenolic compounds in apple fruits

Abstract

Thirty-nine phenolic compounds were analysed using ultra high performance liquid chromatography (UHPLC) coupled with diode array and accurate mass spectrometry detection using electrospray ionisation (DAD/ESI-am-MS). Instrumental parameters such as scan speed, resolution, and mass accuracy were optimised to establish accurate mass measurements. The method was fully validated in terms of model deviation (r2>0.9990), range (typically 10–3500ngg−1), intra/inter-day precision (<6% and <8%, respectively) and accuracy (typically 100±10%). The mass accuracy of each selected phenolic compound was below 1.5ppm. The results confirmed that the UHPLC-DAD/ESI-am-MS method developed here was convenient and reliable for the determination of phenolic compounds in apple extracts.