Abstract
Abstract By using a relatively new liquid phase of moderate polarity (OV-225, Supelco Inc., Bellefonte, Pa.), we have significantly improved the gas-liquid chromatographic analyses of certain steroid hormones from human plasma. This liquid phase has afforded us with nearly complete separation of the free steroids from contaminating organic impurities on the GLC column, thus increasing the accuracy of our analyses due to the Gaussian nature of the recorded chromatographic peaks. Hence, the need for preparing chemical derivatives of these steroids prior to GLC has been eliminated.
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