Abstract
ABSTRACTA rapid micropropagation protocol for Nyctanthus arbor-tristis using nodal segments of the mature plant has been developed. The sterilized nodal segments were cultured on Murashige and Skoog (MS) medium supplemented with 3.0 mg.L–1 6-benzylaminopurine (BAP). This medium combination regenerated 9.0 ± 0.17 shoots from each explant. The shoots were multiplied (41.0 ± 0.24 shoots per explant per culture vessel) by repeated subculturing of freshly induced shoots on MS medium at lower concentrations (1.0 mg.L–1) each of BAP and Kinetin (Kn) and 0.5 mg.L–1 indole-3 acetic acid (IAA). The shoots were rooted (17.0 ± 1.42 roots per shoot) in vitro on half-strength MS medium containing 3.0 mg.L–1 α-naphthalene acetic acid (NAA). The rooted plantlets hardened in the greenhouse with 89% rate of survival.
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