Abstract

A new high-performance liquid chromatographic (HPLC) method for the rapid separation of photosynthetic pigments of higher plants is reported. The method separates zeaxanthin from lutein with a resolution power (Rs) of 1.77. This method uses only two isocratic phases and employs a 10-cm C18 column with a flow rate of 2 mL/min. Consequently, simple HPLC systems can be used for analyses of the pigments, which can be chromatographed in a total time of 13 min. Routine and rapid analysis of the xanthophyll pigments, which were recently implicated in the photoinhibition of photosynthesis, can be achieved with this method.

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