Abstract
An improved normal phase high performance liquid chromatographic (NP-HPLC) method was developed for simultaneous quantification of eight vitamin E isomers (α-, β-, γ- and δ-tocopherols and α-, β-, γ- and δ-tocotrienols) and γ-oryzanol in rice. A complete separation of all compounds was achieved within 25 min using an Inertsil ® CN-3, SIL-100A 5 μM (4.6 mm × 250 mm) column and an isocratic elution system of hexane/isopropanol/ethylacetate/acetic acid (97.6:0.8:0.8:0.8, v/v/v/v) at a flow rate varying from 0.7 to 1.5 mL min −1. A linear correlation coefficient ( r 2 > 0.99) and high reproducibility were obtained at concentrations ranging 0.05–10 μg mL −1 for vitamin E isomers and 0.5–500 μg mL −1 for γ-oryzanol. This method proved to be rapid, accurate and reproducible.
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