Abstract
Acrylic fibres are frequently encountered as physical evidence in casework in the Northern Ireland Forensic Science Laboratory. Separation and analysis of the basic dyes, used in the garment industry for the dyeing of acrylic fibres, was carried out by isocratyic HPLC with a narrow bore column. The HPLC eluent used was a mixture of methanol and aqueous ammonium acetate solution (pH 9.76) in a ratio of 9:1. The sensitivity of the system is such that the dyes extracted from 0.2 cm of a black acrylic fibre could be detected using three injections on a single channel detector at wavelengths of 400, 500 and 600 nm (for yellow, red and blue dye components respectively).
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