Abstract
A modification of the Bennett and Xie chronic constriction injury model of peripheral painful neuropathy was developed in rats. Under tribromoethanol anesthesia, a single ligature with 100% cotton glace thread was placed around the right sciatic nerve proximal to its trifurcation. The change in the hind paw reflex threshold after mechanical stimulation observed with this modified model was compared to the change in threshold observed in rats subjected to the Bennett and Xie or the Kim and Chung spinal ligation models. The mechanical threshold was measured with an automated electronic von Frey apparatus 0, 2, 7, and 14 days after surgery, and this threshold was compared to that measured in sham rats. All injury models produced significant hyperalgesia in the operated hind limb. The modified model produced mean ± SD thresholds in g (19.98 ± 3.08, 14.98 ± 1.86, and 13.80 ± 1.00 at 2, 7, and 14 days after surgery, respectively) similar to those obtained with the spinal ligation model (20.03 ± 1.99, 13.46 ± 2.55, and 12.46 ± 2.38 at 2, 7, and 14 days after surgery, respectively), but less variable when compared to the Bennett and Xie model (21.20 ± 8.06, 18.61 ± 7.69, and 18.76 ± 6.46 at 2, 7, and 14 days after surgery, respectively). The modified method required less surgical skill than the spinal nerve ligation model.
Highlights
Neuropathic pain can persist after nerve damage has been resolved and can be characterized as hypersensitivity to stimuli, the presence of abnormal sensations and a nociceptive response to non-noxious stimuli (1)
Various experimental models can reproduce the neuropathic pain observed in human disease, and the ideal experimental model should result in reproducible sensory deficits, such as allodynia, hyperalgesia and spontaneous pain, over an extended period of time
The chronic constriction injury (CCI) model was the first experimental model used (3), and involves 4 loose chromic gut ligatures placed around the sciatic nerve
Summary
Neuropathic pain can persist after nerve damage has been resolved and can be characterized as hypersensitivity to stimuli (hyperalgesia), the presence of abnormal sensations (hyperpathia) and a nociceptive response to non-noxious stimuli (allodynia) (1). The spinal nerve ligation (SNL) model (5) involves a tight ligation of the L5 and L6 spinal nerves with silk thread. This model produces postoperative behaviors that indicate the presence of neuropathic pain with minimal variability, but requires a high level of surgical skill. We describe a slight modification of the CCI method for producing painful peripheral mononeuropathy in rats. This modified model produces results with little variability when compared to results from the Bennett and Xie CCI model and requires less surgical skill than the Kim and Chung SNL model
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