Abstract
AbstractAn improved enzyme‐linked immunosorbent assay (ELISA) for the determination of southern bean mosaic virus (SBMV) with linear sweep voltammetry based on a new system of p‐aminophenol (PAP)‐H2Q2‐horseradish peroxidase (HRP) has firstly been developed. The enzymatic product 3‐[(4‐hydroxyphenyl)amino]‐4‐(2‐amino‐5‐hydroxvphenyl)‐6‐[(4‐hydroxyphenyl)imino]‐2,4‐cyclohexadiene‐1‐one, produced from the oxidation of PAP with H2O2 catalyzed by HRP, yielded a sensitive linear sweep voltammetric response at −0.45 V (vs. SCE) in Britton‐Robinson (BR) buffer solution. Based on the voltammetric peak, HRP can be measured with a detection limit of 0.4 mU/L and a linear range of 1.0–1.0×102 mU/ L. The detection limit for the SBMV is 8.0 ng/mL and the highest dilution ratio for the detection of infected leaf sap is 1: 1.5×105.
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