Abstract
IT is the general practice among virologists to use 10 per cent normal serum saline as the diluent of choice for the preparation of virus emulsions. In keeping with this practice, horse-sickness vaccine consists essentially of suitably prepared emulsions of selected virus strains in 10 per cent normal horse serum diluted in the same fluid to contain per dose an adequate number of infecting doses of these strains. Since the efficacy of the vaccine is dependent entirely upon the injection of living virus, it is essential that the diluting fluid should be one which favours longevity of the virus. Of a number of media tried, the 10 per cent horse serum saline was found to be the most desirable (Alexander, personal communication).
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