Abstract
Instruments for the measurement of tissue and cell respiration have steadily moved, since the introduction of Warburg's convenient manometer, in the direction of smaller volume and greater sensitivity. Gerard and Hartline1 took advantage of the greater stability afforded by reducing the tissue chamber of a volumeter to capillary dimensions (0.5 to 1.2 mm diameter) and having this inside the relatively large “differential” chamber. Index drop movements, followed with an ocular micrometer, were consistent over 5-minute intervals, even when corresponding to volume changes of about 0.01 cmm. The diver technique, introduced by Linderstrom-Lang,2, 3 is of the same order of sensitivity and consistency; and the electrical method4 gives promise of superior performance.We have further developed the capillary method so that it is convenient to follow the respiration of 10 tissue samples at once, and it is possible to measure absolute gas volume changes of 0.001 cmm, minute by minute, with an error of some 8% minute ...
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