Abstract
Abstract A LC method using amperometric electrochemical detection has been developed for the quantitation of the adenosine regulating agent acadesine (AICA-riboside) in human plasma. Ultrafiltrates of plasma were chromatographed under isocratic conditions on a reverse-phase (C18) LC column using a mobile phase of 1.5% methanol in phosphate buffer at pH 6.3. The column eluant was monitored with a working electrode potential of +750 mV vs Ag/AgCl. Using a 250 μL, sample of plasma for analysis the method has a validated limit of quantitation (LOQ) for acadesine of 6.25 ng/mL with a run-time of 12 minutes/sample. This new method provides a 20-fold decrease in the detection limit for acadesine compared to a previous procedure which employed UV detection.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
