An important role of interleukin-10 in counteracting excessive immune response in HT-29 cells exposed to Clostridium butyricum

Quanxin Gao,Tianxing Wu,Lili Qi,Jinbo Wang

doi:10.1186/1471-2180-12-100

Abstract

BackgroundClostridium butyricum has become increasingly important in preventing and treating intestinal inflammation. In the intestine it may increase the resistance of the gut to pathogen invasion via inducing the secretion of anti-inflammatory cytokines. Interleukin 10 (IL-10) plays a central role in preventing certain inflammatory diseases by down-regulating inflammatory cascades. In a previous study, we observed that the level of IL-10 mRNA was modulated by C. butyricum. The aim of this study was to investigate whether C. butyricum achieves its beneficial effects through IL-10.ResultsWe treated HT-29 cells with anti-IL-10 (IL-10 antibody) or siIL-10 (IL-10 small interfering RNA) to disrupt IL-10. In both cases, the effects of C. butyricum-induced NF-κB activation and IL-8 expression were enhanced. We also found that neutralization or knockdown of IL-10 could induce apoptosis and necrosis of HT-29 cells treated with C. butyricum compared with control cells.ConclusionsThese findings show that IL-10 serves an important role in C. butyricum-mediated immune protection, and in host recognition of C. butyricum.

Highlights

Clostridium butyricum has become increasingly important in preventing and treating intestinal inflammation
Neutralization of Interleukin 10 (IL-10) released by HT-29 cells enhances the effects of C. butyricum-induced NF-κB activation and IL-8 expression Our previous study demonstrated that C. butyricum could induce HT-29 cells to release low levels of proinflammatory cytokines, which is similar to other probiotics such as Lactobacilli [15]
We have shown that C. butyricum can induce HT-29 cells to secrete IL-10

Summary

Introduction

Clostridium butyricum has become increasingly important in preventing and treating intestinal inflammation. In the intestine it may increase the resistance of the gut to pathogen invasion via inducing the secretion of anti-inflammatory cytokines. We observed that the level of IL-10 mRNA was modulated by C. butyricum. The aim of this study was to investigate whether C. butyricum achieves its beneficial effects through IL-10. Probiotic bacteria are live microorganisms which are beneficial to the host organism, and can exert health benefits beyond those of inherent basic nutrition. A recent study indicates that the use of probiotics is rapidly advancing from the field of nutrition towards therapeutic applications [1]. Probiotics have proven useful in preventing and treating diarrhea. Immune modulation and inhibition of excessive immune response and inflammation are proposed to be mechanisms of action of probiotics [4,5]

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