Abstract
Aminopropyltrimethoxysilane (APTMS)-functionalized zinc oxide (ZnO) nanorods and carboxylated graphene nanoflakes (c-GNF) were used in acomposite that was electrophoretically deposited on anindium tin oxide (ITO) coated glass substrate. Themodified ITO electrodes were characterized using various microscopic and spectroscopic techniques which confirm the deposition of theAPTMS-ZnO/c-GNF composite. The electrodes have been used for the covalent immobilization of an Escherichia coli O157:H7 (E. coli)-specific DNA prob. Impedimetric studies revealed that the genesensor displays linearresponse in a wide range of target DNA concentration (10-16M to 10-6M) with a detection limit of 0.1 fM. The studies on thecross-reactivity to other water-borne pathogens show that the bioelectrodeis highly specific. Graphical abstractSchematic illustration for fabrication of nucleic acid biosensor for E. coli DNA detection using anITO electrodemodified with siloxane-functionalized zinc oxide (ZnO) nanorods and carboxylated graphene nanoflakes (c-GNFs).
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