Abstract
Immunoassay plays an important role in the early screening and diagnosis of diseases. The use of electrochemical methods to realize the label-free, specific and rapid detection of antigens has attracted extensive attention from researchers. In this study, we realized the function of immunosensing and detection by lithography, the interdigitated gold electrode on the polyethylene naphthalate (PEN) membrane. Then, the gold electrode was biofunctionalized and the characterization was verified by atomic force microscopy, which was finally for the detection of mice IgG. This immunosensor has a low detection limit, with a broad linear detection range of 0.01–10 ng/mL. The results show that the electrochemical impedance sensor made of metal electrodes based on PEN flexible materials is suitable for immunoassay experiments. If this method could be proved by further studies, broad application prospects can be seen in routine immunoassays.
Highlights
Immunodiagnosis is a method to detect various diseases, based on the specific immune response between antigen and antibody [1,2]
The signal is transmitted from one electrode to another through the medium between the two electrodes, and the signal is read through the electrochemical workstation
Prior to performing the immunoassay experiment, as shown in the electrode schematic diagram of Figure 1b, three test areas were selected from the 3 × 3 interdigital electrode array test area
Summary
Immunodiagnosis is a method to detect various diseases, based on the specific immune response between antigen and antibody [1,2]. The immunodiagnostic methods include: enzyme-linked immunosorbent assay (ELISA) [3], immunofluorescence technology, radioimmunoassay (RIA), immunocolloid gold labeling (ICS), chemiluminescence immunoassay (CLIA) [4,5], etc. CLIA and ELISA are widely used, especially in clinical diagnosis and experimental research [6]. They have become the mainstream of immunodiagnostic technology and have a important position in modern medicine. The electrochemical immunosensor provide the possibilities for label-free detection, detecting electrical signals instead of optical signals. They have been widely applied in biological detections at different scales, including but not limited to, organs, tissues, cells and biomolecules
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