Abstract
Using formalin-fixed, paraffin-embedded sections, indirect immunoperoxidase studies were performed on 3 autopsied and 1 biopsied cases of herpes simplex virus (HSV) encephalitis. Specific reaction was observed in all 4 cases. Distinct stain was observed both in neurons and in glial cells. Electron microscopic studies performed on the same material revealed HSV particles stained with electron-dense reaction products. The advantages and disadvantages of the use of formalin-fixed, paraffin-embedded material in immunoperoxidase studies of viral disease are discussed.
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