Abstract
There was no consistent increase with time in the total activity of extracellular polygalacturonase (PG), relative to mycelial dry weight, when Botrytis cinerea was grown on a modified Czapek Dox liquid medium containing galacturonic acid or glucose. However, preparative isoelectric focusing identified a peak of activity between pH 3.7 and 6.3 only when the fungus was grown in the presence of galacturonic acid. This activity was attributed to the induction of exo-PGs (exo-PGI and/or exo-PGII). Antisera raised against one of two endo-PGs (endo-PGI) reacted positively and specifically with endo-PGI and endo-PGII on Western blots, and both of these isozymes were present in Western blots when the fungus was grown in the presence of glucose or galacturonic acid after 48 h in vitro. Plate-trapped antigen-ELISA confirmed that the endo-PGs were constitutively expressed.
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