Abstract
Antigen sharing amongst gastro-intestinal nematodes viz., Haemonchus contortus, Oesophagostomum columbianum and Bunostomum trigonocephalum was evaluated by inhibition ELISA. Two antigens namely soluble extract antigen (SEA) and gut integral membrane antigen (GIMA) were prepared and hyper immune sera were raised in rabbits against SEA of three referral nematodes. Based on inhibition ELISA, it was observed that the SEA of H. contortus had 36.4 and 28.87% cross-reactivity with inhibited sera of B. trigonocephalum and O. columbianum respectively whereas, the GIMA showed 29.2 and 25.1% crossreactivity with B. trignocephalum and O. columbianum . The SEA of O. columbianum exhibited 34.3 and 22.0% and the GIMA revealed 27.3 and 22.8% cross-reactivity with B. trigonocephalum and H. contortus respectively. The SEA of B. trigonocephalum showed 24.8 and 34.2% and the GIMA exhibited 21.48 and 30.25% crossreactivity with O. columbianum and H. contortus respectively. Further, it was concluded that H. contortus showed a high degree of antigen sharing with B. trigonocephalum than O. columbianum .
Highlights
Parasitic nematodes belonging to the order strongylida represent diverse groups of helminthes with variable economic significance in small ruminants (Jasmer & Mc Guire, 1996)
Parasitic gastro-enteritis caused by H. contortus, O. columbianum and B. trigonocephalum constitute an important group of nematodes and they adversely affect both wool and milk production in animals (Sood, 1981; Knox, 1998)
Assessment of serological cross reactivity amongst referral gastro-intestinal nematodes was made by inhibition enzyme linked immuno sorbent assay (ELISA), to elucidate the reactivity patterns of homologous antigen and antibody system modified in the interface of a cross-reacting heterologous parasite moiety
Summary
Parasitic nematodes belonging to the order strongylida represent diverse groups of helminthes with variable economic significance in small ruminants (Jasmer & Mc Guire, 1996). Parasitic gastro-enteritis caused by H. contortus, O. columbianum and B. trigonocephalum constitute an important group of nematodes and they adversely affect both wool and milk production in animals (Sood, 1981; Knox, 1998). Mortality may arise as an important cause of economic loss, while moderate infection frequently causes stunted growth leading to premature culling of affected animals (Sykes 1994; Sykes & Coop, 2001). Despite the increasing evidence of cross-reactivity among helminths has been reported, the information on strongylid nematodes is, limited (Cuquerella et al, 1993, 1994; Molina et al, 1999). The present investigation was undertaken to elucidate the extent of antigens shared amongst the three gastro-intestinal nematodes namely H. contortus, O. columbianum and B. trigonocephalum using inhibition ELISA
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