Abstract
Experimental silicosis was produced by injecting guinea pigs intratracheally with silica suspensions. Pathological changes consistent with silicosis were noted at autopsy nine months after injection. Many serological tests such as precipitation, passive hemagglutination, complement fixation, and passive cutaneous anaphylaxis were performed on sera from these animals, but none was successful in demonstrating tissue-reactive autoantibodies. It was found that the use of latex particles sensitized with crude lung extracts could not be used to demonstrate tissue-reactive antibodies. It was shown that such particles actually detect rheumatoid factor like antibodies (antiglobulin antibodies). A modification of the antiglobulin consumption test employing lung connective tissue as antigen was developed and made available to study the tissue antibodies in guinea pigs. This modification was quite sensitive in the detection of lung-reactive antibodies in the experimental animals. The development of this serological technique should permit immunological studies of an experimental model of silicosis to be conducted more easily and under more controlled conditions in the future.
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