Abstract
1. 1. The procedures in preparing antigens of the slime mold Dictyostelium discoideum for antibody production in rabbits and for electrophoretic separation are described. 2. 2. The technique of preparing agar plates for immunoelectrophoretic analysis of the antigens is described. 3. 3. The numbers of antigens or specific combining groups synthesized or eliminated during the transition of the slime mold from the vegetative amebas to the mature spores were determined. 4. 4. The numbers of antigens which vegetative amebas, migrating pseudoplasmodia, and mature spores share were determined by absorption of the antiserum. 5. 5. The possibility that the anterior and posterior regions of a migrating pseudoplasmodium differ in their antigenic constitution is discussed. 6. 6. It is suggested that differentiation of the slime mold from the vegetative amebas to the mature sorocarp is accompanied by the destruction of antigens and by the synthesis or change in specificity of other antigens.
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