Abstract
Immunochemical techniques were applied to the investigation of the in vivo distribution of the Parotin subunit, extracted from bovine parotid glands, which decreases serum calcium. The results were as follows.1) Using as an antigen the bioactive Parotin subunit, purified and isolated from bovine parotid glands, specific antibodies to this antigen were produced.2) Using a radioimmunoassay method, these antibodies were found to have a high titer, and in cross-reactivity and Ouchterlony tests as well as immunoelectrophoresis, were shown to be specific for the Parotin subunit.3) Using the PAP enzyme-antibody staining technique, strong staining reactions to these antibodies were found also on the duct cell of bovine, guinea pig and human parotid and submandibular glands as well as sublingual and minor salivary glands. In addition, similarities were observed in pancreatic and gastric glandular and mucosal epithelium. Moreover, staining reactions to these antibodies were also detected in the tumor cells of the duct cell or the hyperkeratic epithelial cell on pleomorphic adenoma adenocarcinoma etc. of human salivary glands. Serum Parotin subunit labeled with 125I was distributed in liver and kidney of guinea pig.
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