An Illumination Compensation for Microscope Images Based on Forgetting Factor

Abstract

In DNA ploidy analysis technology, in order to eliminate the measurement error of DNA relative content caused by uneven illumination and real-time changes in illumination, this article proposes a method of microscope illumination compensation based on forgetting factor. Firstly, multiple images under the microscope are captured, and the cell pixels in these images are cut off by the block threshold segmentation. Secondly, the illumination deviation template library is constructed through the method of illumination deviation template construction based on cumulative background filling proposed in this article. On this basis, a method of microscope illumination compensation with forgetting factor is proposed, and illumination compensation is completed through this method. Experiments show that the method proposed in this article has a good illumination compensation effect on images with uneven illumination for the lack of blank glass slides. Meanwhile, the real-time change of illumination also leads to a poor illumination compensation effect. The method proposed in this article makes the method illumination compensation more stable and accurate, thereby effectively improving the measurement accuracy of DNA ploidy analysis technology.