Abstract
Hardy buds of Azalea spp. were subjected to a sublethal heat stress of 45°C for 2 hours. Two hours after treatment the low temperature exotherms (LTE's) of the primordia were determined by differential thermal analysis (DTA). The LTE's of buds exposed to heat stress were either fewer in number and/or occurred at higher temperatures than in controls. Visual examination of the flower buds following DTA confirmed that treated buds were killed at higher temperatures than control buds. Cold hardy buds which have been pretreated as described also have lower resultant hardiness compared to controls as evidenced by controlled freezing experiments. These studies suggest the presence of an ice barrier within the buds of the plants which avoid freezing by supercooling, and that the barrier is partially overcome by sublethal heat stress.
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