An ic-ELISA and Immunochromatographic Strip Assay for the Detection of 2,4-D in Bean Sprouts and Cabbage

Abstract

The compound 2,4-dichlorophenoxyacetic acid (2,4-D) is a synthetic plant growth regulator, which is widely used in agricultural production. Consequently, it is necessary to establish a rapid and sensitive detection method to monitor its use and prevent the environmental and human health problems caused by overuse. In this study, a monoclonal antibody (mAb) 2D5 against 2,4-D was prepared, and based on it, an ic-ELISA and an immunochromatographic assay (ICS) were developed for rapid detection. The 50% inhibitory concentration (IC50) of the mAb against 2,4-D was 1.7 ng mL-1 and the mAb showed no cross-reactivity with other plant growth regulators with similar structures to 2,4-D. Under optimum conditions, the ICS showed a visual detection limit (vLOD) of 20 ng mL-1 and a cut-off value of 200 ng mL-1 in bean sprouts, and cabbage samples gave the same results. The quantitative detection showed the linear detection ranges for bean sprouts and cabbage samples were 6.3–73.0 and 5.4–132.6 ng mL-1, respectively. The ICS was successfully applied to detect 2,4-D in bean sprouts and cabbage samples and achieved good recoveries ranging from 94.7% to 104.3% with a coefficient of variation (CV) less than 8.3%. Hence, the ICS provides a sensitive, efficient and reliable detection method, which has broad application prospects in the detection of a large number of samples.