Abstract
The existence of a fungus with the ability to nucleate ice formation in supercooled water was revealed. The fungus was isolated from the gut of larvae of the rice stem borer, Chilo suppressalis Walker, and from rice seedlings which were host plants of this insect. The fungus was identified as a Fusarium sp. on the basis of its morphology. Ice-nucleating activity, at around −5°C, was detected in the mycelial suspension of the fungus and also in the culture filtrate. The presence of the exogenous ice-nucleating active fungus in the gut and on the body surface caused an elevation in crystallization temperature of the larvae.
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