An HPLC-FLD method to measure ochratoxin A in teff (Eragrostis tef) and wheat (Triticum spp.) destined for the local Ethiopian market

Abstract

Wheat (Triticum spp.) is among the top cereal grains in terms of production and consumption in Ethiopia. It can be typically infected with ochratoxin A (OTA) producing fungi both in the field and during storage resulting in animal and human health problems. While there is a wealth of information on the presence of OTA in wheat, the incidence of OTA in teff (Eragrostis tef), a cereal grain produced and consumed traditionally in Ethiopia and Eretria, remains insufficient. The purpose of this study was to develop and validate a high performance liquid chromatography (HPLC) method for OTA analysis in teff and wheat flours and to analyse the characteristic distribution of OTA in teff and wheat flours samples destined for local consumption in Ethiopia using a survey-approach. Wheat and teff flour samples were examined for OTA contamination. OTA was detected in 20% of the teff flour samples and in 50% of the wheat flour samples with median contamination levels of 1.04 μg/kg (limit of detection (LOD) = 0.78 μg/kg) and 7.23 μg/kg (LOD = 0.58 μg/kg) respectively. The validated method for OTA detection and quantification in teff and wheat using HPLC meets the European Union performance criteria for OTA (EC 2006/401) and the Eurachem Guideline validation requirements. An insight into the occurrence of OTA in teff is very valuable because the cereal might provide a lower OTA risk alternative for wheat in Ethiopia. In addition, for the world market, an insight into the occurrence of OTA in teff is important in the light of an increasing interest in teff as a gluten free cereal.