Abstract
A method for the quantitative analysis of pyruvate and acetate groups in polysaccharides has been developed, using HPLC on a cation exchange resin in the protonated form and u.v. detection at 210 nm. Procedures for release of acetate and pyruvate from polysaccharides are described. In the case of xanthan gum, most of the pyruvate is removed if the sample is autoclaved in water during preparation, and about 25% is lost if autoclaved in 0·1 m sodium chloride solution.
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