Abstract
An extracellular galactoxylomannan (GalXM) composed of d-Gal (34.0%), d-Xyl (26.6%) and d-Man (31.0%), and a small amount of l-Ara (4.0%) and d-Glc (4.4%) has been isolated from culture medium of acapsulated mutant of Cryptococcus laurentii by ethanol precipitation and gel filtration. Phosphorylated polymer of M w ∼ 75 000 contained 90% carbohydrates, 3.9% phosphorus and 5.3% proteins. Results of chemical and spectroscopic studies showed a highly branched structure of GalXM with a 1,6-linked mannopyranosyl–galactopyranosyl backbone (∼44%) branched predominantly at C-2 and C-3 of mannosyl, and C-3 of galactosyl residues by side chains terminated mainly by xylosyl and mannosyl residues, and to a less extent by arabinosyl and glucosyl ones.
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More From: International Journal of Biological Macromolecules
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