An exploratory cohort study of serum estradiol, testosterone, osteoprotegerin, interleukin-6, calcium, and magnesium as potential biomarkers of cervical spondylosis

Abstract

BackgroundExploration of biomarkers for debilitating diseases such as cervical spondylosis is important to revolutionize clinical diagnosis and management of such conditions. The study aimed to determine the correlation between neck pain and disability and serum levels of interleukin-6 (IL-6), osteoprotegerin (OPG), estradiol (E2), testosterone (TES), calcium (Ca), and magnesium (Mg) among individuals with symptomatic cervical spondylosis.MethodsThis study was a cohort design. The participants were new referrals to two Nigerian physical therapy clinics. Participants’ neck pain intensity (PI), neck disability index (NDI), IL-6, OPG, E2, TES, Ca, and Mg were measured at baseline and after 13 weeks of follow-up. Data were analyzed using descriptive statistics, independent samples t test, Pearson’s correlation, and multiple linear regression.ResultsForty individuals aged 52.40 ± 8.60 years participated in the study. Women had significantly higher levels of IL-6 (t = − 2.392, p = 0.026), OPG (t = − 3.235, p = 0.005), E2 (t = − 6.841, p = 0.001), but lower TES (t = 17.776, p = 0.001). There were no significant sex differences in PI and NDI. There were significant correlations between PI and OPG (r = 0.385, p < 0.001), NDI and OPG (r = 0.402, p < 0.001), and IL-6 (r = 0.235, p = 0.036). Significant predictors of PI were OPG (β = 0.442, p < 0.001) and E2 (β = − 0.285, p = 0.011), and NDI were OPG (β = 0.453, p < 0.001), E2 (β = − 0.292, p = 0.005), and IL-6 (β = 0.225, p = 0.024).ConclusionHigh serum levels of IL-6 and OPG were associated with cervical spondylosis severity. However, high serum levels of E2 and TES correlated with lesser severity. Moreover, TES inversely correlated with the proinflammatory cytokines.