Abstract
In the systems, investigated, the adsorption of specific antibody by cells or by tissue residues conformed over a limited range to an expression similar to von Krogh's equation. This resulted in a straight line plot of the adsorption data. Alternatively, the adsorption data could be plotted as probits. For direct adsorption of antibodies, satisfactory agreement with this expression could be demonstrated only with purified antibody. In contrast, tests for antibody present in supernatants obtained following a direct adsorption gave results that agreed well, even when unpurified antisera were used. The systems studies were immune hemolysis of sheep red cells, immune cytolysis of mouse thymocytes, and binding of radio-labeled globulins to stromata of a mouse ovarian tetratoma. Preliminary evidence suggested that the above findings with antibody might represent a special case of a more general adsorption phenomenon.
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