Abstract
Methotrexate (MTX) is an antineoplastic agent, which increases the level of reactive oxygen species (ROS) and decreases the level of antioxidants. Lycopene, is a potent antioxidant, which is used because of its protective effect against tissue damage. The aim of this study was to determine the effect of lycopene on ovarian MTX-induced injury in rats. Rats (n = 36) were randomly divided into 3 equal groups: a group with MTX only (MG, n = 12), a group with lycopene and MTX (LMG, n = 12), and a healthy control group (HCG, n = 12). Then, malondialdehyde (MDA), myeloperoxidase (MPO) and total glutathione (tGSH) levels and histopathological findings were examined in the ovaries of rats. Apart from the histopathological and biochemical evaluation, the reproductive performance of the experimental groups was also examined. Our study demonstrated that, in ovarian tissues of rats administered MTX, there was a decrease in the levels of tGSH, while MDA and MPO were increased, but it is observed that these ratios are reversed in the LMG (p < 0.05). It also has been proven that a single, high-dose use of MTX causes infertility in female rats, prolongs the gestation period and reduces the number of offspring. Lycopene pretreatment ameliorates the MTX induced ovarian injury and infertility in rats through its antioxidative activities.
Highlights
Methotrexate (MTX) is a chemotherapeutic agent that is a folic acid antagonist
In ovarian tissues of rats administered MTX, there was a decrease in the levels of tGSH, while MDA and MPO were increased, but it is observed that these ratios are reversed in the lycopene and mg/kg of MTX (LMG) (p < 0.05)
When the other 2 groups were compared with the MG, MPO was significantly increased in the MG (7.1 ±0.7 U/g of protein), but the value in the LMG (2.8 ±0.6 U/g of protein) was close to the healthy control group (HCG) (1.9 ±0.7 U/g of protein) (p < 0.05)
Summary
Methotrexate (MTX) is a chemotherapeutic agent that is a folic acid antagonist This agent, which acts by inhibiting the activity of dihydrofolate reductase, is frequently used in the treatment of autoimmune and malignant diseases.[1] It has been found that this agent, which is frequently used in ectopic and molar pregnancy treatments, increases the level of free oxygen radicals and pro-inflammatory molecules.[2] It is gonadotoxic and decreases the number of ovarian follicles. Lycopene, is a potent antioxidant, which is used because of its protective effect against tissue damage
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