An experimental study on the endothelium protective effect of adenosine-lidocaine cardioplegia on canine coronary artery

Abstract

Objective By observing the changes in levels of nitric oxide (NO),endothelin (ET) and circulating endothelial cells (CEC),and that in functions of coronary vasomotor,the author discusses the protective effects of adenosine-Lidocaine cardioplegia on endothelial cells of canine coronary artery and its possible mechanism.Methods 12 mongrel dogs were randomly divided into two groups:experimental group with Adenosine-lidocaine cardioplegia (AL group,n =6) and the control group with ST.Thomas Ⅱ cardioplegia (K group,n =6).Measured the changes in levels of NO,ET and CEC on three time points:before operation (T1),1 h after heart re-beat (T2) and 2 h after heart re-beat (T3),observed the changes in functions of coronary vasodilation,and analyzed the coronary ultrastructure by using electron microscope.Results There were no statistical differences between the two groups in T1.On the time point of T3,NO in plasma of group K has been significantly decreased than that of group AL (P ＜ 0.05).ET has been increased significantly (P ＜ 0.05),so as CEC (P ＜ 0.05).The maximum percentage of coronary vasodilation in group K is (29.13 ± 8.24)％ which is lower than that of AL group (68.52 ± 11.32)％ (P ＜ 0.01).Electron microscopy results show that the damage of coronary vascular endothelial cell ultrastructure of group K is more serious than that of group AL.Conclusion The protective effect of Adenosine-Lidocaine cardioplegia on canine coronary vascular endothelial cell is superior to that of ST.Thomas Ⅱ cardioplegia. Key words: Adenosine ; Lidocaine; Cardiopulmonary bypass; Coronary artery ; Circulating endothelial cell