Abstract
Objective To explore the possibility of isolating and purifying Schwann cells from newborn mouse fat tissues. Methods Adipose tissue was harvested from 5 to 7 day-old neonatal GFP mice. The tissue was digested with 0.2% compound collagenase NB4 to obtain cells. The derived cells were then cultured in lowglucose DMEM with 10% FBS for 24 hours. 0.1% compound collagenase NB4 was used to purify Schwann cells by differential isolation once every 48 hours for two times total. p75 fluorescent immunostaining was performed to determine the purity of Schwann cells. Results The cells isolated from mouse groin subcutaneous fat tissue were Schwann cells. The purity of Schwann cells reached as high as 90% after two time purification.Conclusion Purified Schwann cells can be isolated from mouse fat tissue, which provides a new source for seed cells for tissue-engineered nerves. Key words: Adipose tissue; Schwann cells; Cell separation; Purification
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