Abstract
Obese rats manifested the characteristics of insulin resistance after high.fat-diet feeding for 20 weeks.Double immunofluorescence showed that endocannabinoid receptor 1(CBIR)was greatly expressed in pancreatic β-cels.The results of immunohistochemistry and real-time quantitative PCR revealed that CBIR protein (6 002.8±855.5 vs 3 712.8±769.6,P<0.01)and mRNA(16.7 folds of control group,P<0.05)expressions in higll-fat group were higher than those of control group,suggesting a role of CB1 R in high-fat-diet induced insulin resistance. Key words: Obesity; Endocannabinoid receptor 1; Rats; Sprague-Dawley; Islets of Langerhans; Indirect immunofluorescence
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