Abstract
Objective To observe the effect of Schwann cells attached by avidin-biotin binding system (ABBS) on regeneration of tissue engineered nerve.Methods Sciatic nerve 10 mm defect model was created in 40 Wister rats that were divided into four groups.In group A the defect was repaired by autologous nerve graft.In group B the defect was repaired with Poly( L-lactic acid-co-epsilon-caprolactone) [ P(LLA-CL) ]scaffold.In group C the defect was brideged with P(LLA-CL) scaffold filled with Schwann cells.In group D the defect was bridged by P(LLA-CL) scaffold attached with Schwann cells by avidin-biotin.The sciatic functional index (SFI),nerve conduction velocity,the number of nerve fibers and thickness of myelin sheath were measured 3 months postoperatively.Results There was no difference between groups C and D.The results of both groups were better than those of the no Schwann cell group B,but worse than those of the autograft group A.Conclusion ABBS does not have adverse effects on nerve regeneration using tissue-engineered nerve in the in vivo application and is a reliable method to attach cells to biomaterials. Key words: Biotin; Nerve regeneration; Avidin; Ploy(L-lactide-co-epsilon-caprolactone)
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