Abstract
Abstract Evidence has been presented in the literature suggesting that certain interracial phenomena may produce cellular damage in culture operations. In an earlier study of bubble-driven fluid motions in a sparged bioreactor reported by Glasgow et al. (1992), some energetic transitions associated with the bubble formation process were observed at sieve plate spargers. These transitions, involving both the active hole location and mode, were found to be independent of gas supply pressure fluctuations but quite sensitive to gas chamber volume. In the present study, five different experimental techniques have been employed in an effort to better characterize these transitions. Macrovideography and photographic flow visualization were initially used to record, classify, and count the transitions and it was discovered that frequency of transition was a qualitatively reproducible function of superficial gas velocity. A light-scattering experiment was then devised in which fluctuations in received light intens...
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