Abstract
To assess induction effects of Chlamydia pneumoniae (Cpn) on lung cancer in rats. A lung cancer animal model was developed through repeated intratracheal injection of Cpn (TW-183) into the lungs of rats, with or without exposure to benzo(a)pyrene (Bp). Cpn antibodies (Cpn-IgA, -IgG, and -IgM) in serum were measured by microimmunofluorescence. Cpn-DNA or Cpn-Ag of rat lung cancer was detected through polymerase chain reaction or enzyme-linked immunosorbent assay. The prevalence of Cpn infection was 72.9% (35/48) in the Cpn group and 76.7% (33/43) in the Cpn plus benzo(a)pyrene (Bp) group, with incidences of lung carcinomas in the two groups of 14.6% (7/48) and 44.2% (19/43), respectively (P-values 0.001 and <0.001 compared with normal controls). A rat model of lung carcinoma induced by Cpn infection was successfully established in the laboratory for future studies on the treatment, prevention, and mechanisms of the disease.
Highlights
Lung cancer is one of the most common malignant tumors threatening health and life, causing more deaths than any other malignant disease
We verified our previous research on Chlamydia pneumoniae (Cpn) and lung cancer linkage using serum and tumor tissue from patients by designing a Cpn infection-induced lung tumor model in rats with or without benzo (a)pyrene (Bp), a carcinogen found in cigarettes
Polymerase chain reaction (PCR) Lung cancer tissues of rats positive for serum Cpn antibody were stored at low temperature
Summary
Lung cancer is one of the most common malignant tumors threatening health and life, causing more deaths than any other malignant disease. Recent clinical epidemiological studies have suggested that an intimate relationship exists between Chlamydia pneumoniae (Cpn) infection and lung cancer incidence. Cpn infection has been identified as a risk factor for lung cancer (Zhan et al, 2011). Cpn has become one of the hottest research points of the disease. Most scholars used clinical epidemiological research or serum gathering to determine the relationship between Cpn and lung cancer. Animal models of Cpn infections inducing lung cancer have not yet been reported. We verified our previous research on Cpn and lung cancer linkage using serum and tumor tissue from patients by designing a Cpn infection-induced lung tumor model in rats with or without benzo (a)pyrene (Bp), a carcinogen found in cigarettes. The results of the present study provide a new perception and method for lung cancer nosogenesis and therapy
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