An experimental consideration of the improved (1974) Kelsy-Sykes test for bactericidal effect of disinfectants

Abstract

In 1974, Kelsey and Maurer proposed the improved Kelsey-Sykes test as an evaluation method for bactericidal effect of all kinds of disinfectant. We examined the necessity of culturing test organism with the fixed special broth, preparing organism population and using the special container called the universal container proposed in this test. We compared these test results with the phenol coefficient method used usually as an evaluation method for bactericidal effect of disinfectants, and investigated its availability and experimental procedure: the results are as follows. 1) There was not difference between growth medium according to K-S test (Bacto Synthetic Broth A.O.A.C. Code No. 0352) and our nutrient broth (Nissui Nutrient Broth Lot No. 065210) in growth support for the test organisms. 2) There was not difference in test results of bactericidal effect between the standard hard water and whole broth culture used as test organisms suspension or organism suspension with the pellicle of P. aeruginosa and without one. 3) The result using the usual operating test tube was the same as when the universal container was used. 4) The procedure of K-S test was complex in comparison with the PC test method, but was able to presume the usual use-dilution. The K-S test had an advantage in this point.