Abstract
Objectives: To research experimental bacteriostasis on the Banxiaxiexin decoction and 7 kinds of single taste Traditional Chinese Medicine (TCM) for Helicobacter pylori resistant strains in vitro. Methods: Collecting specimens of gastric mucosa in patients with upper gastrointestinal disease, separated and identified 8 strains of Hp resistant strains, the E-test methods of metronidazole and clarithromycin, drug resistance status of amoxicillin, selected eight strains of clinical drug resistance strains; Using liquid dilution method to determine the Banxiaxiexin decoction and 7 kinds of single Chinese medicine clinical drug-resistant strains of 8 strains of antibacterial effect in vitro. Results: Banxiaxiexin decoction MIC is 0.31 mg/ml, MIC of Radix scutellariae is 0.09 mg/ml, Rhizoma coptidis MIC 0. 16 mg/ml, Glycyrrhizin realness MIC 0. 31 mg/ml, Ginseng MIC 0. 75 mg/ml, there are four of seven kinds of single taste Traditional Chinese Medicine of TCM has bacteriostatic action, the order of antibacterial strength: Scutellaria baicalensis>Coptis chinesis>Glycyrrhizin realness >Ginseng, three kinds of Pinellia, Jujube and dry Zingibe in vitro for H. P ylori no inhibition, the method of MIC with liquid dilution in this decoction were determined, the decoction of resistant isolates average MIC isolates was 0.20±0.09mg/ml, the results showed that this decoction have stronger antibacterial effect on eight kinds of drug resistant strains in vitro. Banxiaxiexin decoction and 4kinds of single taste TCM have stronger antibacterial effect on drug resistant strains in vitro. Conclusion: Banxiaxiexin decoction and 4 kinds of single TCM have strong bacteriostatic effect on the bacteria resistance of Helicobacter pylori strains in vitro, therefore, we suggest that this decoction could be used as an effective method in the treatment of resistant HP associated gastritis.
Highlights
Helicobacter pylori is the cause of chronic active gastritis and duodenal ulcer
From the perspective of Traditional Chinese Medicine to study the prevention and control drug resistant strains has opened up a new way, Banxiaxiexin decoction from zhang zhongjing in Chinese han dynasty [4], Shang-han lun of prescriptions for Pinellia, Scutellaria baicalensis, Zingiber, Ginseng, Glycyrrhiza uralensis, Coptis chinesis, Jujube, the function may be modulated the cold and heat balance, eliminated swelling of the liver disease and dissipated nodules, Indicating on cold and heat mixed in the ruffian Syndrome as well as abdomen with swelling of the liver disease, but abdominal fullness without pain, or vomiting, bowel diarrhea, and yellowish greasy tongue
Drug sensitivity test: we have collected fresh Helicobacter pylori (HP) from pure culture for 48-72 hours and fully grind it into sterile saline, adjust the concentration to 2 × 108cfu / ml, dipped the sterile cotton swab in the bacterial solution, evenly coated the whole agar surface, stand, slightly dry, took the E-test strip containing antibiotics and laid it flat on the agar surface (1 / 90 mm plate), 37°C, and cultured in microaerobic environment for 72 hours, the results showed that the minimum inhibitory concentration (MIC) was the scale at the junction of the ellipsoidal bacteriostatic ring and the test strip
Summary
It is given priority to antibiotic combination scheme of Hp infection with effective treatment of western medicine [1], but along with the increase in antibiotic use, side effects and the resistant strains are more and more [2,3]. From the perspective of Traditional Chinese Medicine to study the prevention and control drug resistant strains has opened up a new way, Banxiaxiexin decoction from zhang zhongjing in Chinese han dynasty [4], Shang-han lun of prescriptions for Pinellia, Scutellaria baicalensis, Zingiber, Ginseng, Glycyrrhiza uralensis, Coptis chinesis, Jujube, the function may be modulated the cold and heat balance, eliminated swelling of the liver disease and dissipated nodules, Indicating on cold and heat mixed in the ruffian Syndrome as well as abdomen with swelling of the liver disease, but abdominal fullness without pain, or vomiting, bowel diarrhea, and yellowish greasy tongue. American Journal of Internal Medicine 2020; 8(3): 138-142 curative effects on treating Helicobacter pylori related gastric disease in clinical observation of the decoction, especially resistant Helicobacter pylori cases, but the specific mechanism is unclear, we have researched experimental bacteriostasis on the decoction and 7 kinds of single taste Traditional Chinese Medicine (TCM) for Helicobacter pylori resistant strains in vitro, the purpose is to explore the decoction and 7 kinds of single Chinese medicine resistance mechanism of H. pylori resistant strains, could be provided the objective basis for the prevention and treatment of H. pylori resistant strains
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