Abstract
Potato tissue culture has been used to regenerate microtubers. The technology represents a convenient aid for teaching the control and development of underground plant storage organs Regeneration of potato (Solatium tuberosum L.) microtubers in tissue culture is used as a model simulating the developmental physiology of underground storage organ formation. Two exercises are described: ji) induction of microtubers on tissue culture-derived single nodal expiants, incubated an an agar-solidified Murashige and Skoog basal medium devoid erf plant growth regulators, but supplemented with high sucrose concentrations; and (ii) prevention of sucrose-mediated microtuberization by adding gibberellic acid (GA3) to the medium. The objectives of these experiments were to (a) illustrate that, from a developmental standpoint, underground storage organs are shortened, thickened stems; and (b) show that sucrose participates in tuber induction in a growth regulator-like mode of action.
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