An examination on the effect of entomopathogenic in vitro Metarhizium anisopliae and Numeraea rileye on Spodoptera litura

Abstract

Grayak worm (Spodoptera litura) is one of the important pests because it has a broad host range including soybeans, peanuts, cabbage, sweet potatoes and potatoes. So far, farmers still depend on chemical insecticides that are considered practical in the application and the results are quickly visible. The use of entomopathogen (insect pathogen) is an environmentally-friendly biological control method. Metarhizium anisopliae and Numeraea rileye are secondary metabolite-producing fungal species that act as neuromuscular toxins and can cause depolarization of insect muscle membranes. This study aimed to examine the effectiveness of entomopathogenic in-vitro Metarhizium anisopliae and Nomuraea rileyi against (Spodoptera litura). The study was conducted in the microbiology laboratory of University of Muhammadiyah Malang from November 2017 to February 2018. The study used a simple randomized block design and the treatment consisted of applications of Metarhizium anisopliae and Nomuraea rileyi with populations of 105, 106, 107, 108 which repeated 3 times. The data were analyzed using the analysis of variants and then examined by the real and different types of test. The results showed that there were significant differences between the examined treatments. Nomuraea Rileyi with a population of 108 is the best biological agent for controlling Spodoptera litura pests.Grayak worm (Spodoptera litura) is one of the important pests because it has a broad host range including soybeans, peanuts, cabbage, sweet potatoes and potatoes. So far, farmers still depend on chemical insecticides that are considered practical in the application and the results are quickly visible. The use of entomopathogen (insect pathogen) is an environmentally-friendly biological control method. Metarhizium anisopliae and Numeraea rileye are secondary metabolite-producing fungal species that act as neuromuscular toxins and can cause depolarization of insect muscle membranes. This study aimed to examine the effectiveness of entomopathogenic in-vitro Metarhizium anisopliae and Nomuraea rileyi against (Spodoptera litura). The study was conducted in the microbiology laboratory of University of Muhammadiyah Malang from November 2017 to February 2018. The study used a simple randomized block design and the treatment consisted of applications of Metarhizium anisopliae and Nomuraea rileyi with populations o...